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iron ii acetate tetrahydrate is an effective synthetic reagent for determining iron oxidation rates by phototrophic Fe(II)-oxidizing bacteria under varying conditions of pH, temperature, and light. The resulting data will help to better understand the physiological limitations of this metabolism and therefore the possible role of phototrophic Fe(II)-oxidizers in the deposition of BIFs on early earth.
Process for the preparation of iron ii acetate tetrahydrate
The chemical preparation of iron ii acetate is achieved through a reaction of a solution containing 0.2 M to 0.8 M oxalic acid and 10-100 parts by weight of acetic acid with 1 part by weight of the iron hydroxide. The reaction is performed preferably at a temperature of 80-100deg C and in pH 0.5-2.5 to minimize the content of impurities in the final product.
Rates of Fe(II) oxidation by Rhodobacter ferrooxidans strain SW2 under various conditions at varying Fe(II) concentrations (a) in filtered and (b) nonfiltered medium. The results indicate that at a high Fe(II) concentration, in particular in the presence of higher amounts of Fe(II) minerals, the dissolution rate of the Fe(II) minerals limits the overall oxidation rate.
This might also be due to a direct oxidation of the Fe(II) minerals by the phototrophic bacteria in the periplasm. However, further experiments are needed to distinguish the contribution of dissolved Fe(II) from the poorly crystalline Fe(II) minerals versus directly oxidized Fe(II) in the periplasm of phototrophic bacteria. This will improve our understanding of the interactions between phototrophic Fe(II)-oxidizers and iron reducers in modern environments.